Nadin Almosnid
Middle Tennessee State University, USA
Title: In vitro cytotoxicity and initial safety evaluation of extracts from 16 plants used in Yao ethno medicine
Biography
Biography: Nadin Almosnid
Abstract
Cancer is the second cause of death due to the limited treatment options. Because of the side effects and drug resistance of the current chemotherapy, there is an urgent need to develop new chemotherapeutic agents. Based on an ethno pharmacological survey, sixteen plant species widely used in the Medicines of Yao ethnic group in China, which is a special branch of traditional Chinese medicine (TCM), were collected and 64 plant extracts were prepared from these plants. An in vitro cytotoxicity screening was conducted with a panel of four human cancer cell lines namely, lung cancer A549, breast cancer BT20 and MCF-7, bone cancer U2OS. Besides, their possible toxicity was evaluated using two normal human cell lines, lung HPL1A and breast HMEC. A potent activity (IC50 < 5 µg/mL) was observed for ethanol extract from Melaleuca leucadendra Linnagainst against U2OS cells. The most potent extract was acetate extract from Elephantopus scaber against all tested cancer cells (BT20, A549, U2S, and MCF7) with IC50 range between 3.1-24 µg/mL. However, most of the antitumor behaviours were against U2OS cell with IC50 49.3-4 µg/ml. The selectivity index of the active samples varied from 0 to > 100. Additionally, extracts that showed the anti-proliferation activity exhibited the ability of inducing apoptosis in U2OS cells. This study provides in vitro evidence for the traditional use of these ethno medicinal plants, and validates the safety of these plant extracts.